Human EGF-containing fibulin-like extracellular matrix protein 2 (EFEMP2) ELISA Kit | KTE61951

Human EGF-containing fibulin-like extracellular matrix protein 2 (EFEMP2) ELISA Kit | KTE61951 | Gentaur UK, US & Europe Distribution

Application: This Human EGF-containing fibulin-like extracellular matrix protein 2 (EFEMP2) ELISA Kit employs a two-site sandwich ELISA to quantitate EFEMP2 in samples. An antibody specific for EFEMP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEFEMP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EFEMP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EFEMP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human EGF-containing fibulin-like extracellular matrix protein 2 microplate
• Human EGF-containing fibulin-like extracellular matrix protein 2 standard
• Human EGF-containing fibulin-like extracellular matrix protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human EGF-containing fibulin-like extracellular matrix protein 2 (EFEMP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human EFEMP2. No significant cross-reactivity or interference between Human EFEMP2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: A large number of extracellular matrix proteins have been found to contain variations of the epidermal growth factor (EGF) domain and have been implicated in functions as diverse as blood coagulation, activation of complement and determination of cell fate during development. FBLN4 contains four EGF2 domains and six calcium-binding EGF2 domains. This gene is necessary for elastic fiber formation and connective tissue development. Defects in this gene are cause of an autosomal recessive cutis laxa syndrome.EFEMP1 is likewise expressed in a wide range of adult and fetal tissues. In contrast to EFEMP1, however, EFEMP2 was not significantly overexpressed in senescent or quiescent fibroblasts, suggesting a diversity of function within this EGF-like domain subfamily.

Alternative Names: EFEMP2; FBLN4; MBP1; UPH1; fibulin 4; mutant p53 binding protein 1

Search name: EFEMP2; FBLN4; MBP1; UPH1; fibulin 4; mutant p53 binding protein 1

Tag: EFEMP2