Human Enteropeptidase (PRSS7) ELISA Kit | AE25597HU

Human Enteropeptidase (PRSS7) ELISA Kit | AE25597HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: PRSS7

Alternative Name: ENTK; MGC133046; enterokinase|enteropeptidase

Application: ELISA

Range: Request Information

Sensitivity: Request Information

Intra-Assay: ≤4.2%

Inter-Assay: ≤7.7%

Recovery: 0, 9

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PRSS7 in samples. An antibody specific for PRSS7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRSS7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRSS7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSS7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Enteropeptidase is an enzyme involved in human digestion. It is produced by cells in the duodenum wall, and is secreted from duodenum's glands, the crypts of Lieberkühn, whenever ingested food enters the duodenum from the stomach. Enteropeptidase has the critical job of turning trypsinogen (a zymogen) to trypsin, indirectly activating a number of pancreatic digestive enzymes. Enteropeptidase is a serine protease enzyme (EC 3.4.21.9) which converts inactive trypsinogen into active trypsin by cleavage at the C-terminal end of the sequence. It consists of a disulfide-linked 82–140 kDa heavy chain which anchors enterokinase in the intestinal brush border membrane and a 35–62 kDa light chain which is the catalytic subunit.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .