Description
Human EPHA5 (Ephrin Type A Receptor 5) ELISA Kit | ELK7778 | Gentaur UK, US & Europe Distribution
Alternative Names: TYRO4; CEK7; EHK1; HEK7; Brain-specific kinase; EPH homology kinase 1; EPH-like kinase 7
Species: Human
Assay Type: Sandwich
Sensitivity: 0.112 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Neuro science
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ephrin Type A Receptor 5 (EPHA5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ephrin Type A Receptor 5 (EPHA5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ephrin Type A Receptor 5 (EPHA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ephrin Type A Receptor 5 (EPHA5) in the samples is then determined by comparing the OD of the samples to the standard curve.