Human Excitatory amino acid transporter 4 (SLC1A6) ELISA Kit | AE19078HU

Human Excitatory amino acid transporter 4 (SLC1A6) ELISA Kit | AE19078HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: SLC1A6

Alternative Name: EAAT4; MGC33092; MGC43671; excitatory amino acid transporter 4

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.129 ng/mL

Intra-Assay: ≤5.3%

Inter-Assay: ≤9.2%

Recovery: 1, 08

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate SLC1A6 in samples. An antibody specific for SLC1A6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC1A6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC1A6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC1A6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: EAAT4 is expressed predominantly in the cerebellum, has high affinity for the excitatory amino acids L-aspartate and L-glutamate. When stimulated by these amino acids, EAAT4 conducts chloride ions.Excitatory amino acid transporters (EAATs) in the central nervous system maintain extracellular glutamate concentrations below excitotoxic levels and may limit the activation of glutamate receptors. The transport activity encoded by EAAT4 has high apparent affinity for L-aspartate and L-glutamate, and has a pharmacologic profile consistent with previously described cerebellar transport activities. In Xenopus oocytes expressing EAAT4, L-aspartate and L-glutamate elicited a current predominantly carried by chloride ions. This chloride conductance was not blocked by components that block endogenous oocyte chloride channels.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .