Human Exosome complex exonuclease RRP44 (DIS3) ELISA Kit | KTE62049

Human Exosome complex exonuclease RRP44 (DIS3) ELISA Kit | KTE62049 | Gentaur UK, US & Europe Distribution

Application: This Human Exosome complex exonuclease RRP44 (DIS3) ELISA Kit employs a two-site sandwich ELISA to quantitate DIS3 in samples. An antibody specific for DIS3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDIS3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DIS3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DIS3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Exosome complex exonuclease RRP44 microplate
• Human Exosome complex exonuclease RRP44 standard
• Human Exosome complex exonuclease RRP44 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Exosome complex exonuclease RRP44 (DIS3) ELISA Kit has high sensitivity and excellent specificity for detection of Human DIS3. No significant cross-reactivity or interference between Human DIS3 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: By randomly sequencing clones obtained from a size-fractionated brain cDNA library, Nagase et al. (1999) cloned DIS3, which they designated KIAA1008. The deduced 928-amino acid protein shares 44.5% identity with the mitotic control protein Dis3 of S. pombe. PCR-ELISA detected low to moderate expression in all tissues and brain regions tested, with highest expression in testis and moderate expression in kidney, ovary, corpus callosum, spinal cord, and fetal brain. By sequence analysis of chromosome 13q21-q22 to identify a breast cancer susceptibility gene, Rozenblum et al. (2002) identified DIS3. The deduced protein contains 958 amino acids. Northern blot analysis detected ubiquitous expression.

Alternative Names: DIS3; DKFZp667L1817; EXOSC11; FLJ10484; KIAA1008; MGC33035; RP11-342J4.3; RRP44; bA555G22.1; dis3p; 2810028N01Rik; DIS3 mitotic control; exosome component 11; mitotic control protein dis3 homolog

Search name: DIS3; DKFZp667L1817; EXOSC11; FLJ10484; KIAA1008; MGC33035; RP11-342J4.3; RRP44; bA555G22.1; dis3p; 2810028N01Rik; DIS3 mitotic control; exosome component 11; mitotic control protein dis3 homolog

Tag: DIS3