Human F13 (Coagulation Factor ⅩⅢ) ELISA Kit | E-EL-H0762

Human F13 (Coagulation Factor ⅩⅢ) ELISA Kit | E-EL-H0762 | Gentaur UK, US & Europe Distribution

Type: Sandwich

Assay Time: 3.5h

Sensitivity: 15pg/mL

Detection Range: 25~1600pg/mL

UniProt ID: P00488 (F13A1)&P05160 (F13B)

Target Name: F13

Target Synonym: Factor XIII, fibrin stabilizing factor , F13, Laki-Lorand factor

Test Principale: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human F13. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human F13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human F13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human F13. You can calculate the concentration of Human F13 in the samples by comparing the OD of the samples to the standard curve.

Specifivity: This kit recognizes Human F13 in samples. No significant cross-reactivity or interference between Human F13 and analogues was observed.

Research Areas: Cancer, Cardiovascular, Tags and Cell Markers

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.