Human FAAH (Fatty Acid Amide Hydrolase) ELISA Kit | ELK7632

Human FAAH (Fatty Acid Amide Hydrolase) ELISA Kit | ELK7632 | Gentaur UK, US & Europe Distribution

Alternative Names: FAAH-1; Anandamide amidohydrolase 1; Oleamide hydrolase 1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.58 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Amide Hydrolase (FAAH) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Fatty Acid Amide Hydrolase (FAAH) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Amide Hydrolase (FAAH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fatty Acid Amide Hydrolase (FAAH) in the samples is then determined by comparing the OD of the samples to the standard curve.