Human FBLN3 (Fibulin 3) ELISA Kit | ELK3553

Human FBLN3 (Fibulin 3) ELISA Kit | ELK3553 | Gentaur UK, US & Europe Distribution

Alternative Names: EFEMP1; DHRD; DRAD; FBNL; MLVT; MTLV; S1-5; EGF-Containing Fibulin-Like Extracellular Matrix Protein 1; Fibrillin-Like; Extracellular protein S1-5

Species: Human

Assay Type: Sandwich

Sensitivity: 0.64 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: serum, plasma, urine and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibulin 3 (FBLN3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Fibulin 3 (FBLN3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibulin 3 (FBLN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibulin 3 (FBLN3) in the samples is then determined by comparing the OD of the samples to the standard curve.