Human FGF3 (Fibroblast Growth Factor 3) ELISA Kit | ELK2940

Human FGF3 (Fibroblast Growth Factor 3) ELISA Kit | ELK2940 | Gentaur UK, US & Europe Distribution

Alternative Names: HBGF3; INT2; Heparin-binding growth factor 3; Murine Mammary Tumor Virus Integration Site (v-int-2) Oncogene Homolog; INT-2 Proto-Oncogene Protein; Proto-oncogene Int-2

Species: Human

Assay Type: Sandwich

Sensitivity: 5.7 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Cytokine

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 3 (FGF3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Fibroblast Growth Factor 3 (FGF3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 3 (FGF3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibroblast Growth Factor 3 (FGF3) in the samples is then determined by comparing the OD of the samples to the standard curve.