Human Fibronectin type-III domain-containing protein C4orf31 (C4orf31) ELISA Kit | KTE62297

Human Fibronectin type-III domain-containing protein C4orf31 (C4orf31) ELISA Kit | KTE62297 | Gentaur UK, US & Europe Distribution

Application: This Human Fibronectin type-III domain-containing protein C4orf31 (C4orf31) ELISA Kit employs a two-site sandwich ELISA to quantitate C4orf31 in samples. An antibody specific for C4orf31 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyC4orf31 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C4orf31 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C4orf31 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Fibronectin type-III domain-containing protein C4orf31 microplate
• Human Fibronectin type-III domain-containing protein C4orf31 standard
• Human Fibronectin type-III domain-containing protein C4orf31 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Fibronectin type-III domain-containing protein C4orf31 (C4orf31) ELISA Kit has high sensitivity and excellent specificity for detection of Human C4orf31. No significant cross-reactivity or interference between Human C4orf31 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: NDNF is evolutionarily well conserved, being present in invertebrate animals. Multiple cysteines are conserved between species and secondary structure prediction shows that NDNF is mainly composed of beta-strands.NDNF is a glycosylated, disulfide-bonded secretory protein that contains a fibronectin type III domain. NDNF promoted migration and growth and elicited neurite outgrowth of mouse hippocampal neurons in culture. NDNF also protected cultured hippocampal neurons against excitotoxicity and amyloid beta-peptide toxicity. NDNF was exclusively expressed in the brain and spinal cord. NDNF was expressed by neurons and not by astrocytes. NDNF expression was observed in the neurons during development.NDNF is a novel neurotrophic factor derived from neurons that may be useful in the treatment of neuronal degeneration diseases and nerve injuries.

Alternative Names: C4orf31; FLJ23191; hypothetical protein LOC79625

Search name: C4orf31; FLJ23191; hypothetical protein LOC79625

Tag: C4orf31