Human Flavin reductase (BLVRB) ELISA Kit | AE61679HU

Human Flavin reductase (BLVRB) ELISA Kit | AE61679HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: BLVRB

Alternative Name: BVRB; FLR; MGC117413; SDR43U1; flavin reductase (NADPH) |short chain dehydrogenase/reductase family 43U; member 1

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.124 ng/mL

Intra-Assay: ≤3.6%

Inter-Assay: ≤6.9%

Recovery: 0, 96

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate BLVRB in samples. An antibody specific for BLVRB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBLVRB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BLVRB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BLVRB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: The final step in heme metabolism in mammals is catalyzed by the cytosolic biliverdin reductase enzymes A and B. BLVRB is found predominantly in fetal liver. BLVRA is a major component of human adult liver and is identical to the enzyme previously reported as biliverdin reductase.The FLR cDNA, which appeared to be identical to the BLVRB cDNA, encodes a deduced 206-amino acid protein. Western blot analysis of rat tissues showed highest levels of FLR in the erythrocytes and liver. Northern blot analysis detected a 1.1-kb transcript in human tissues. that flavins are the effective electron acceptor of the enzyme and purified FLR to homogeneity.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .