Human G antigen 7 (GAGE7) ELISA Kit | KTE62390

Human G antigen 7 (GAGE7) ELISA Kit | KTE62390 | Gentaur UK, US & Europe Distribution

Application: This Human G antigen 7 (GAGE7) ELISA Kit employs a two-site sandwich ELISA to quantitate GAGE7 in samples. An antibody specific for GAGE7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGAGE7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GAGE7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GAGE7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human G antigen 7 microplate
• Human G antigen 7 standard
• Human G antigen 7 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human G antigen 7 (GAGE7) ELISA Kit has high sensitivity and excellent specificity for detection of Human GAGE7. No significant cross-reactivity or interference between Human GAGE7 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Many human tumors express antigens that are recognized in vitro by cytolytic T lymphocytes (CTLs) derived from the tumor-bearing patient. The MAGE (melanoma-specific antigen) and GAGE (G antigen) gene family members encode such antigens. To screen for genes associated with cancer progression, Chen et al. (1998) performed differential display PCR using sublines of the nontumorigenic, prostate-specific antigen (PSA) -secreting cell line LNCaP that differed in androgen sensitivity and metastatic potential. They isolated 2 members of the GAGE family, GAGEB1 and GAGE7. GAGE7 encodes a 117-amino acid polypeptide that contains 3 potential casein kinase phosphorylation sites, 2 potential protein kinase C phosphorylation sites, and 1 potential N-myristoylation site. Levels of GAGE7 mRNA were not correlated to the degree of tumorigenicity of prostate cancer cell lines.

Alternative Names: GAGE7; CT4.7; GAGE-7; cancer/testis antigen family 4; member 7

Search name: GAGE7; CT4.7; GAGE-7; cancer/testis antigen family 4; member 7

Tag: GAGE7