Human GAL12 (Galectin 12) ELISA Kit | ELK5304

Human GAL12 (Galectin 12) ELISA Kit | ELK5304 | Gentaur UK, US & Europe Distribution

Alternative Names: LGALS12; GRIP1; Lectin, Galactoside-Binding Soluble 12; Galectin-related inhibitor of proliferation

Species: Human

Assay Type: Sandwich

Sensitivity: 12.9 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galectin 12 (GAL12) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Galectin 12 (GAL12) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galectin 12 (GAL12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Galectin 12 (GAL12) in the samples is then determined by comparing the OD of the samples to the standard curve.