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Human GAPDH Activity Assay Kit II - K2047

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SKU:
26-K2047-100-GEN
Availability:
Usually shipped in 5 working days
NULL584.00

Description

K2047 | Human GAPDH Activity Assay Kit II DataSheet

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH; EC 1.2.1.12) catalyzes the conversion of Glyceraldehyde-3-Phosphate (GAP) to 1, 3-Bisphosphate Glycerate (BPG) and plays a key role in glycolysis. The enzyme is involved in cellular processes such as apoptosis, membrane trafficking, iron metabolism and nuclear translocation. GAPDH is a stable metabolic enzyme and is constitutively expressed. Therefore, it is considered as a housekeeping gene. Deregulation of GAPDH activity is associated with cell proliferation and carcinogenesis. Accurate detection of GAPDH activity is important for diagnosing diseases and studying normal cellular physiology. ’s Human GAPDH Activity Assay Kit II provides a simple and sensitive method for monitoring GAPDH activity in various sample types. In this assay, GAPDH catalyzes GAP into BPG and an intermediate, which then reacts with the developer to form a colored product that is measured at 450 nm. Our high-throughput adaptable assay can detect GAPDH activity as low as 10 mU/mL in a variety of samples.

Alternate Name : Glyceraldehyde-3-Phosphate Dehydrogenase,Human GAPDH Activity Kit II,Human GAPDH,GAPDH Activity Assay Kit II,Human GAPDH Activity Kit,Human GAPDH Activity Assay,EC 1.2.1.12,GAPDH Activity Assay,GAPDH Activity Assay Kit,Human GAPDH Activity Assay Kit,GAPDH Assay,GAPDH Activity Kit

Tag Line : For monitoring GAPDH activity

Summary : ’s Human GAPDH Activity Assay Kit II provides a simple and sensitive method for monitoring GAPDH activity in various sample types. In this assay, GAPDH catalyzes GAP into BPG and an intermediate, which then reacts with the developer to form a colored product that is measured at 450 nm. Our high-throughput adaptable assay can detect GAPDH activity as low as 10 mU/mL in a variety of samples.

Detection Method : Absorbance (OD 450 nm)

Sample Type : Animal tissues: Liver, Heart etc.,Cell culture: Adherent or suspension cells

Species Reactivity : All

Applications : Measurement of GAPDH activity in various tissues and cells,Analysis of glycolysis and pentose phosphate pathways

Features and Benefits : Sensitive method for monitoring GAPDH activity in various sample types,Simple, rapid & convenient

 

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Additional Information

Storage Conditions:
-20°C
Shipping Conditions:
Gel Pack
Shelf life:
12 months
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