Human GJb1 (Gap Junction Protein Beta 1) ELISA Kit | ELK3976

Human GJb1 (Gap Junction Protein Beta 1) ELISA Kit | ELK3976 | Gentaur UK, US & Europe Distribution

Alternative Names: CMTX1; CX32; GJ-B1; Connexin 32; Charcot-Marie-Tooth Neuropathy, X-Linked; GAP junction 28 kDa liver protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.108 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Tumor immunity;Infection immunity;Neuro science;Bone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gap Junction Protein Beta 1 (GJb1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Gap Junction Protein Beta 1 (GJb1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gap Junction Protein Beta 1 (GJb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gap Junction Protein Beta 1 (GJb1) in the samples is then determined by comparing the OD of the samples to the standard curve.