Description
Human GJb1 (Gap Junction Protein Beta 1) ELISA Kit | ELK3976 | Gentaur UK, US & Europe Distribution
Alternative Names: CMTX1; CX32; GJ-B1; Connexin 32; Charcot-Marie-Tooth Neuropathy, X-Linked; GAP junction 28 kDa liver protein
Species: Human
Assay Type: Sandwich
Sensitivity: 0.108 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Tumor immunity;Infection immunity;Neuro science;Bone metabolism
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gap Junction Protein Beta 1 (GJb1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Gap Junction Protein Beta 1 (GJb1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gap Junction Protein Beta 1 (GJb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gap Junction Protein Beta 1 (GJb1) in the samples is then determined by comparing the OD of the samples to the standard curve.