Human GLS1, Recombinant | P1656

Glutaminase (EC 3.5.1.2) is a mitochondrial enzyme that hydrolyzes glutamine producing glutamate and ammonia. GLS is one of the two key enzymes including glutamine synthetase that are responsible for glutamine homeostasis. Since glutamine is a major metabolic substrate involved in gluconeogenesis, its homeostasis is tightly controlled. Glutaminase has tissue-specific roles in multiple organs, with the isoform GLS1 being expressed in kidneys and brain, and GLS2 being expressed in the liver. It is involved in maintenance of acid-base homeostasis by producing ammonia during renal acidosis in kidneys and regulation of glutamate, which acts as a neurotransmitter in the brain. ’s recombinant GLS1 is catalytic domain of the original GLS1, which shows greater activity and excellent stability.

P1656 | Human GLS1, Recombinant DataSheet

Biomolecule/Target :

Synonyms: Glutaminase, cKGA, catalytic domain of KGA, Glutaminase kidney isoform, mitochondrial, KGA, GLS1, GAC, GAM, KGA

Alternates names: Glutaminase, cKGA, catalytic domain of KGA, Glutaminase kidney isoform, mitochondrial, KGA, GLS1, GAC, GAM, KGA

Taglines: A mitochondrial enzyme that hydrolyzes glutamine producing glutamate and ammonia

NCBI Gene ID #:

NCBI Gene Symbol:

Gene Source: E Coli

Accession #: O94925

Recombinant: TRUE

Source:

Purity by SDS-PAGE: ≥ 95% by SDS-PAGE

Assay:

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method):

Biological activity: Biological activity was performed using ’s Glutaminase Activity Assay Kit (Colorimetric) (Cat# K2067-100).

Results:

Binding Capacity:

Unit Definition: One unit of GSL1 is defined as the amount of enzyme that produces 1 umol of glutamate per minute at 37 °C under the assay conditions.

Molecular Weight: 36.7 kDa

Concentration: Lot specific

Appearance: Colorless liquid

Physical form description: Liquid

Reconstitution Instructions:

Amino acid sequence: aa 221-533

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans