Human HBb (Hemoglobin Beta) ELISA Kit | ELK4071

Human HBb (Hemoglobin Beta) ELISA Kit | ELK4071 | Gentaur UK, US & Europe Distribution

Alternative Names: HB-B; HBD; CD113t-C; Beta Globin; Spinorphin

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 2.94 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: serum, plasma and erythrocyte lysates

Assay Lenght: 2h

Research Area: Hematology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hemoglobin Beta (HBb) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hemoglobin Beta (HBb) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hemoglobin Beta (HBb) in the samples is then determined by comparing the OD of the samples to the standard curve.