Human HDL (High Density Lipoprotein) ELISA Kit | ELK1728

Human HDL (High Density Lipoprotein) ELISA Kit | ELK1728 | Gentaur UK, US & Europe Distribution

Alternative Names: High Density Lipoprotein

Species: Human

Assay Type: Sandwich

Sensitivity: 1.1 ng/mL

Standard: 150 ng/mL

Detection Range: 2.35-150 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Nutrition metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Density Lipoprotein (HDL) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to High Density Lipoprotein (HDL) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Density Lipoprotein (HDL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Density Lipoprotein (HDL) in the samples is then determined by comparing the OD of the samples to the standard curve.