Human Helicase ARIP4 (RAD54L2) ELISA Kit | KTE60983

Human Helicase ARIP4 (RAD54L2) ELISA Kit | KTE60983 | Gentaur UK, US & Europe Distribution

Application: This Human Helicase ARIP4 (RAD54L2) ELISA Kit employs a two-site sandwich ELISA to quantitate RAD54L2 in samples. An antibody specific for RAD54L2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAD54L2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAD54L2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAD54L2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Helicase ARIP4 microplate
• Human Helicase ARIP4 standard
• Human Helicase ARIP4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Helicase ARIP4 (RAD54L2) ELISA Kit has high sensitivity and excellent specificity for detection of Human RAD54L2. No significant cross-reactivity or interference between Human RAD54L2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Adrenergic receptors (ARs) include four general types that are found in different target tissues and differ in their affinities and responses to various agonists and antagonists. The coupling of ARs to specific intracellular effectors is mediated through diverse heterotrimeric G proteins. RAD54L2 (RAD54-like 2), also known as ARIP4, HSPC325 or SRISNF2L, is a 1, 467 amino acid nuclear protein belonging to the SNF2/RAD54 helicase family that consists of one helicase ATP-binding domain and a helicase C-terminal domain. RAD54L2 is a DNA helicase that regulates androgen receptor (AR) -dependent transactivation in a promoter-dependent manner. RAD54L2 is posttranslationally sumoylated or phosphorylated upon DNA damage.

Alternative Names: RAD54L2; FLJ21396; FLJ22400; HSPC325; KIAA0809; SRISNF2L; RAD54-like 2

Search name: RAD54L2; FLJ21396; FLJ22400; HSPC325; KIAA0809; SRISNF2L; RAD54-like 2

Tag: RAD54L2