855
Human HIF1aN (Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor) ELISA Kit | ELK4964
- SKU:
- 855-ELK4964-GEN
Description
Human HIF1aN (Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor) ELISA Kit | ELK4964 | Gentaur UK, US & Europe Distribution
Alternative Names: FIH1; Peptide-Aspartate Beta-Dioxygenase; Hypoxia-inducible factor asparagine hydroxylase
Species: Human
Assay Type: Sandwich
Sensitivity: 14.4 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Immune molecule
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor (HIF1aN) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor (HIF1aN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor (HIF1aN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor (HIF1aN) in the samples is then determined by comparing the OD of the samples to the standard curve.