Human HMGB-1 (High Mobility Group Protein B1) CLIA Kit | E-CL-H0997

Human HMGB-1 (High Mobility Group Protein B1) CLIA Kit | E-CL-H0997 | Gentaur UK, US & Europe Distribution

Type: Sandwich

Assay Time: 3.5h

Sensitivity: 7.5pg/mL

Detection Range: 12.5~800pg/mL

UniProt ID: N/A

Target Name: HMGB-1

Target Synonym: HMGB1, HMG1, HMG3, SBP-1, Amphoterin

Test Principale: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HMGB-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HMGB-1. You can calculate the concentration of Human HMGB-1 in the samples by comparing the RLU value of the samples to the standard curve.

Specifivity: This kit recognizes Human HMGB-1 in samples. No significant cross-reactivity or interference between Human HMGB-1 and analogues was observed.

Research Areas: Cancer, Epigenetics and Nuclear Signaling

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.