Human HNRPU (Heterogeneous Nuclear Ribonucleoprotein U) ELISA Kit | ELK3522

Human HNRPU (Heterogeneous Nuclear Ribonucleoprotein U) ELISA Kit | ELK3522 | Gentaur UK, US & Europe Distribution

Alternative Names: hnRNP-U; HNRNPU; SAF-A; U21.1; Scaffold Attachment Factor A

Species: Human

Assay Type: Sandwich

Sensitivity: 0.057 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heterogeneous Nuclear Ribonucleoprotein U (HNRPU) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heterogeneous Nuclear Ribonucleoprotein U (HNRPU) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heterogeneous Nuclear Ribonucleoprotein U (HNRPU), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heterogeneous Nuclear Ribonucleoprotein U (HNRPU) in the samples is then determined by comparing the OD of the samples to the standard curve.