Human Homeobox protein CDX-2 (CDX2) ELISA Kit | KTE62284

Human Homeobox protein CDX-2 (CDX2) ELISA Kit | KTE62284 | Gentaur UK, US & Europe Distribution

Application: This Human Homeobox protein CDX-2 (CDX2) ELISA Kit employs a two-site sandwich ELISA to quantitate CDX2 in samples. An antibody specific for CDX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCDX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CDX2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CDX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Homeobox protein CDX-2 microplate
• Human Homeobox protein CDX-2 standard
• Human Homeobox protein CDX-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Homeobox protein CDX-2 (CDX2) ELISA Kit has high sensitivity and excellent specificity for detection of Human CDX2. No significant cross-reactivity or interference between Human CDX2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Caudal type homeobox transcription factor 2 is a homeobox transcription factor.Cdx2 is the gene that directs early embryogenesis in mice. It is required to form the placenta.Ectopic expression of CDX2 was reported more than 85% of the human patients with Acute myeloid leukemia (AML) . Ectopic expression of Cdx2 in murine bone marrow induced AML in mice and upregulate Hox genes in bone marrow progenitors.This gene (or, more specifically, the equivalent gene in humans) has come up in the proposal by the President's Council on Bioethics, as a solution to the stem cell controversy. According to one of the plans put forth, by deactivating the gene, it would not be possible for a properly organized embryo to form, thus providing stem cells without requiring the destruction of an embryo.

Alternative Names: CDX2; CDX-3; CDX3; caudal type homeo box transcription factor 2; caudal type homeobox transcription factor 2

Search name: CDX2; CDX-3; CDX3; caudal type homeo box transcription factor 2; caudal type homeobox transcription factor 2

Tag: CDX2