Human hs Ab1-42 (high sensitivity Amyloid Beta Peptide 1-42) ELISA Kit | ELK8920

Human hs Ab1-42 (high sensitivity Amyloid Beta Peptide 1-42) ELISA Kit | ELK8920 | Gentaur UK, US & Europe Distribution

Alternative Names: Amyloid Beta Peptide 1-42;

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 1.35 pg/mL

Standard: 300 pg/mL

Detection Range: 4.69-300 pg/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Neuro science

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with high sensitivity Amyloid Beta Peptide 1-42 (hs Ab1-42) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to high sensitivity Amyloid Beta Peptide 1-42 (hs Ab1-42) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of high sensitivity Amyloid Beta Peptide 1-42 (hs Ab1-42) in the samples is then determined by comparing the OD of the samples to the standard curve.