Human HSPA1L (Heat Shock 70kDa Protein 1 Like Protein) ELISA Kit | ELK5549

Human HSPA1L (Heat Shock 70kDa Protein 1 Like Protein) ELISA Kit | ELK5549 | Gentaur UK, US & Europe Distribution

Alternative Names: HSP-A1L; HSPA1-L; HSP70-HOM; Hum70t

Species: Human

Assay Type: Sandwich

Sensitivity: 0.65 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Infection immunity;Developmental science;Pulmonology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 1 Like Protein (HSPA1L) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 1 Like Protein (HSPA1L) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70kDa Protein 1 Like Protein (HSPA1L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 70kDa Protein 1 Like Protein (HSPA1L) in the samples is then determined by comparing the OD of the samples to the standard curve.