Description
Human HTN1 (Histatin 1) ELISA Kit | ELK4651 | Gentaur UK, US & Europe Distribution
Alternative Names: PPB; HIS1; Histidine-rich protein 1; Post-PB protein;
Species: Human
Assay Type: Sandwich
Sensitivity: 0.67 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Metabolic pathway
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histatin 1 (HTN1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Histatin 1 (HTN1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histatin 1 (HTN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histatin 1 (HTN1) in the samples is then determined by comparing the OD of the samples to the standard curve.