Human IGFALS (Insulin Like Growth Factor Binding Protein, Acid Labile Subunit) ELISA Kit | ELK3718

Human IGFALS (Insulin Like Growth Factor Binding Protein, Acid Labile Subunit) ELISA Kit | ELK3718 | Gentaur UK, US & Europe Distribution

Alternative Names: ALS

Species: Human

Assay Type: Sandwich

Sensitivity: 0.66 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Endocrinology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS) in the samples is then determined by comparing the OD of the samples to the standard curve.