Human IgG4 (Immunoglobulin G4) ELISA Kit | ELK2338

Human IgG4 (Immunoglobulin G4) ELISA Kit | ELK2338 | Gentaur UK, US & Europe Distribution

Alternative Names: IGHG4; Ig Gamma-4 Chain C Region; Immunoglobulin Heavy Constant Gamma 4; G4m Marker; Immunoglobulin Gm4

Species: Human

Assay Type: Sandwich

Sensitivity: 0.073 ug/mL

Standard: 10 ug/mL

Detection Range: 0.16-10 ug/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity;Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin G4 (IgG4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Immunoglobulin G4 (IgG4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin G4 (IgG4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin G4 (IgG4) in the samples is then determined by comparing the OD of the samples to the standard curve.