Human IkBa (Inhibitory Subunit Of NF Kappa B Alpha) ELISA Kit | ELK2219

Human IkBa (Inhibitory Subunit Of NF Kappa B Alpha) ELISA Kit | ELK2219 | Gentaur UK, US & Europe Distribution

Alternative Names: NFKBIA; IKB-A; MAD-3; NFKBI; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor, Alpha; Major histocompatibility complex enhancer-binding protein MAD3

Species: Human

Assay Type: Sandwich

Sensitivity: 0.065 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Tumor immunity;Hematology;Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inhibitory Subunit Of NF Kappa B Alpha (IkBa) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Inhibitory Subunit Of NF Kappa B Alpha (IkBa) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inhibitory Subunit Of NF Kappa B Alpha (IkBa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inhibitory Subunit Of NF Kappa B Alpha (IkBa) in the samples is then determined by comparing the OD of the samples to the standard curve.