Human IL-12 (IL-12A & IL-12B) protein, His Tag | PRP100705

Human IL-12 (IL-12A & IL-12B) protein, His Tag | PRP100705 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cytokines

Product Type: Cytokines

Sequence: Amino acid sequence derived from p35 subunit of human IL-12, termed as IL-12A (NP_000873.2) (Met 1-Ser 219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 ; Amino acid sequence derived from p40 subunit of human IL-12, termed as IL-12B (NP_002178.2) (Met 1-Ser 328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.

Activity: Measured by its ability to bind biotinylated recombinant human IL-12RB1 in a functional ELISA. Measured in a cell proliferation assay using Anti-CD28 and Anti-CD3 stimulated PBMC. The ED50 for this effect is typically 0.1-0.6 ng/mL. 2. Measured in a cell proliferation assay using Anti-CD28 and Anti-CD3 stimulated PBMC. The ED50 for this effect is typically 0.1-0.6 ng/mL.

protein Lenght: The recombinant IL-12 heterodimer of human IL-12A/IL-12B comprises 525 (208 + 317) amino acids and has a calculated molecular mass of 60.2 (24 + 36.2) kDa. The apparent molecular mass of rh IL-12 heterodimer is approximately 40 & 43 kDa respectively in SDS-PAGE under reducing conditions.

Preparation: HEK293 Cells

Purity: > 95 % as determined by SDS-PAGE

Formulation: Lyophilized from sterile PBS, pH 7.4

Kit Component: N/A

Features & Benefits : N/A

Molecular Weight: 60.2 (24 + 36.2) kDa

Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL- 12 (IL12A & IL12B Heterodimer) protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Storage Conditions: Lyophilized Human IL-12 (IL-12A & IL-12B) protein, His Tagshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.96% HSA or BSA) . Please prevent freeze-thaw cycles.

Shipping: The product is shipped at ambient temperature.

Background: IL-12 is composed of a bundle of four α helices. It is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40) . The active heterodimer (referred to as 'p70'), and a homodimer of p40 are formed following protein synthesis.IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-γ) and tumor necrosis factor-α (TNF-α) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. T cells that produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes. There also seems to be a link between IL-2 and the signal transduction of IL-12 in NK cells. IL-2 stimulates the expression of two IL-12 receptors, IL-12R-β1 and IL-12R-β2, maintaining the expression of a critical protein involved in IL-12 signaling in NK cells. Enhanced functional response is demonstrated by IFN-γ production and killing of target cells.

Alternative Names: CLMF; CLMF2; IL-12A; IL-12B; IL12; IMD28; IMD29; NFSK; NKSF; NKSF1; NKSF2; P35; Interleukin-12 subunit alpha & Interleukin-12 subunit beta; IL12A & IL12B

Search Names: CLMF; CLMF2; IL-12A; IL-12B; IL12; IMD28; IMD29; NFSK; NKSF; NKSF1; NKSF2; P35; Interleukin-12 subunit alpha & Interleukin-12 subunit beta; IL12A & IL12B

Tag: IL12A & IL12B