Human IL-12β protein, Fc Tag | PRP100018

Human IL-12β protein, Fc Tag | PRP100018 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cytokines

Product Type: Cytokines

Sequence: Amino acid sequence derived from p40 subunit of human IL-12, termed as IL-12β (NP_002178.2) (Met 1-Ser 328) was fused with the Fc region ofhuman IgG1 at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA . Immobilized human IL-12RB1 at 20 μg/ml (100 μl/well) can bind human IL-12B with a linear ranger of 2.56-64 ng/ml.

protein Lenght: The recombinant human IL-12β consists of 544 amino acids and has a predicted molecular mass of 61.4 kDa.

Preparation: HEK293 Cells

Purity: > 85 % as determined by SDS-PAGE

Formulation: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5

Kit Component: N/A

Features & Benefits : N/A

Molecular Weight: 61.4 kDa

Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL12B protein, Fc Tag in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Storage Conditions: Lyophilized Human IL-12β protein, Fc Tagshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.29% HSA or BSA) . Please prevent freeze-thaw cycles.

Shipping: The product is shipped at ambient temperature.

Background: Subunit β of interleukin 12 (also known as natural killer cell stimulatory factor 2, or cytotoxic lymphocyte maturation factor 2, p40) (IL-12B) is a subunit of human interleukin 12. IL-12B/IL-12B is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL-12A. IL-12B/IL-12B is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. IL-12B/IL-12B associates with IL-23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity.

Alternative Names: CLMF; CLMF2; IL-12B; IMD28; IMD29; NKSF; NKSF2; Interleukin-12 subunit beta; IL12B

Search Names: CLMF; CLMF2; IL-12B; IMD28; IMD29; NKSF; NKSF2; Interleukin-12 subunit beta; IL12B

Tag: IL-12β