Human IL-24 protein, His Tag | PRP100299

Human IL-24 protein, His Tag | PRP100299 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cytokines

Product Type: Cytokines

Sequence: Amino acid sequence derived from human IL-24 isoform 3 (NP_001172085.1) (Met 1-Leu 207) was fused with a polyhistidine tag at the C-terminus.

Activity: Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human IL­20 Rα and human IL­20 Rβ. The EC50 for this effect is typically 0.05-0.25 ng/mL.

protein Lenght: The secreted recombinant human IL-24 consists of 166 amino acids and has a predicted molecular mass of 19.5 kDa. As a result of different glycosylation, the apparent molecular mass of rhIL-24 is approximately 35 kDa in SDS-PAGE under reducing conditions.

Preparation: HEK293 Cells

Purity: > 94 % as determined by SDS-PAGE

Formulation: Lyophilized from sterile PBS, pH 7.4

Kit Component: N/A

Features & Benefits : N/A

Molecular Weight: 19.5 kDa

Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL24 protein, His Tag in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Storage Conditions: Lyophilized Human IL-24 protein, His Tagshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.76% HSA or BSA) . Please prevent freeze-thaw cycles.

Shipping: The product is shipped at ambient temperature.

Background: Interleukin-24 (IL-24) also known as Melanoma differentiation-associated gene 7 protein (MDA-7) is a member of the IL-10 family of cytokines. IL-24/MDA-7/IL-24 can induce apoptosis selectively in various cancer cells. Overexpression of IL-24 leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Human IL-24/MDA-7/IL-24 is secreted by activated peripheral blood mononuclear cells and is the ligand for two heterodimeric receptors, IL-22R1/IL-20R2 and IL-20R1/IL-20R2. Northern blot analysis revealed IL-24/MDA-7/IL-24 expression in human tissues associated with the immune system such as spleen, thymus, peripheral blood leukocytes and normal melanocytes. IL-24/MDA-7/IL-24 binding to either its endogenous receptors on human keratinocytes or to ectopically expressed receptors on baby hamster kidney cells leads to activation of the signal transducers and activators of transcription.

Alternative Names: C49A; FISP; IL10B; MDA7; MOB5; ST16; Interleukin-24; IL24

Search Names: C49A; FISP; IL10B; MDA7; MOB5; ST16; Interleukin-24; IL24

Tag: IL-24