Human IL-35 (IL-12A & IL-27B) protein, Fc Tag | PRP100184

Human IL-35 (IL-12A & IL-27B) protein, Fc Tag | PRP100184 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cytokines

Product Type: Cytokines

Sequence: Amino acid sequence derived from human IL-35 complex composed of IL-27B subunit (NP_005746.2) (Met 1-Lys 229) and the mature form of human IL-12 p35 subunit (P29459) (Arg 23-Ser 219) linked by a polypeptide linker was fused with the Fc region of human IgG1 at the C-terminus.

Activity: Measured by its ability to bind biotinylated human IL-6RB-Fch in a functional ELISA.

protein Lenght: The recombinant human IL-35/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 645 amino acids and has a predicted molecular mass of 73.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIL-35/Fc monomer is approximately 90-100 kDa due to glycosylation.

Preparation: HEK293 Cells

Purity: > 85 % as determined by SDS-PAGE

Formulation: Lyophilized from sterile PBS, pH 7.4

Kit Component: N/A

Features & Benefits : N/A

Molecular Weight: 73.4 kDa

Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL-35 (IL12A & IL27B) protein, Fc Tag in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Storage Conditions: Lyophilized Human IL-35 (IL-12A & IL-27B) protein, Fc Tagshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.54% HSA or BSA) . Please prevent freeze-thaw cycles.

Shipping: The product is shipped at ambient temperature.

Background: The novel Ebi3-IL-12α heterodimeric cytokine has been designated interleukin-35 (IL-35), is a member IL-12 family cytokine produced by regulatory T cells (Treg), but not by resting or activated effector T cells (Teff) . IL-35 is a heterodimeric protein composed of IL-12α (P35) and IL-27β chains, which are encoded by two separate genes called IL-12A and EBI3 (Epstein-Barr-virus-induced gene 3) respectively. Ectopic expression of IL-35 confers regulatory activity on naive T cells, whereas recombinant IL-35 suppresses T-cell proliferation. It identify IL-35 as a novel inhibitory cytokine that may be specifically produced by T (reg) cells and is required for maximal suppressive activity. IL-35 has biological activity and able to expand CD4+CD25+ Treg cells, suppress the proliferation of CD4+CD25- effector cells and inhibit Th17 cell polarization. IL-35 has been shown to be constitutively expressed by regulatory T (Treg) cells CD4 (+) CD25 (+) Foxp3 (+) and suggested to contribute to their suppressive activity. IL-35 is a crucial mediator which provokes CD4+CD25+ T cell proliferation and IL-10 generation, another well-known anti-inflammatory cytokine, along with TGFβ cytokine. IL-35 is a cytokine can downregulate Th17 cell development and inhibit autoimmune inflammation. It inhibited the differentiation of Th17 cells in vitro. In vivo, IL-35 effectively attenuated established collagen-induced arthritis in mice, with concomitant suppression of IL-17 production but enhanced IFN-gamma synthesis. Thus, IL-35 is a novel anti-inflammatory cytokine suppressing the immune response through the expansion of regulatory T cells and suppression of Th17 cell development.

Alternative Names: CLMF; IL-12A; IL-35; Interleukin-35; NFSK; NKSF1; P35; Interleukin-27 subunit beta & Interleukin-12 subunit alpha; IL35

Search Names: CLMF; IL-12A; IL-35; Interleukin-35; NFSK; NKSF1; P35; Interleukin-27 subunit beta & Interleukin-12 subunit alpha; IL35

Tag: IL-35