Human IL1e (Interleukin 1 Epsilon) ELISA Kit | ELK4790

Human IL1e (Interleukin 1 Epsilon) ELISA Kit | ELK4790 | Gentaur UK, US & Europe Distribution

Alternative Names: IL1F6; IL36a; FIL1; FIL1 (EPSILON) ; FIL1E; IL-1F6; IL1 (EPSILON) ; Interleukin 36, Alpha; Interleukin 1 Family, Member 6

Species: Human

Assay Type: Sandwich

Sensitivity: 2.7 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Cytokine

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Epsilon (IL1e) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 1 Epsilon (IL1e) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Epsilon (IL1e), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Epsilon (IL1e) in the samples is then determined by comparing the OD of the samples to the standard curve.