Human Integral membrane protein GPR137B (GPR137B) ELISA Kit | KTE62389

Human Integral membrane protein GPR137B (GPR137B) ELISA Kit | KTE62389 | Gentaur UK, US & Europe Distribution

Application: This Human Integral membrane protein GPR137B (GPR137B) ELISA Kit employs a two-site sandwich ELISA to quantitate GPR137B in samples. An antibody specific for GPR137B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGPR137B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPR137B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPR137B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Integral membrane protein GPR137B microplate
• Human Integral membrane protein GPR137B standard
• Human Integral membrane protein GPR137B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Integral membrane protein GPR137B (GPR137B) ELISA Kit has high sensitivity and excellent specificity for detection of Human GPR137B. No significant cross-reactivity or interference between Human GPR137B and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: TM7SF1, a gene that is transcriptionally upregulated during kidney development. RT-PCR detected 3 alternatively spliced TM7SF1 transcripts, which the authors named TM7SF1-long (TM7SF1L), TM7SF1-intermediate (TM7SF1I), and TM7SF1-short (TM7SF1S) . Northern blot analysis detected an approximately 2.4-kb TM7SF1 transcript at highest levels in human kidney and heart, with lower levels detected in brain and placenta. Additional experiments found TM7SF1 expression in spinal cord, caudate nucleus, and putamen. Studies on Wilms tumor samples showed variable TM7SF1 expression, ranging from nearly undetectable levels to an abundant level of expression comparable to that of adult kidney tissue.

Alternative Names: GPR137B; TM7SF1; transmembrane 7 superfamily member 1 (upregulated in kidney)

Search name: GPR137B; TM7SF1; transmembrane 7 superfamily member 1 (upregulated in kidney)

Tag: GPR137B