Human Interferon-inducible protein AIM2 (AIM2) ELISA Kit | KTE60635

Human Interferon-inducible protein AIM2 (AIM2) ELISA Kit | KTE60635 | Gentaur UK, US & Europe Distribution

Application: This Human Interferon-inducible protein AIM2 (AIM2) ELISA Kit employs a two-site sandwich ELISA to quantitate AIM2 in samples. An antibody specific for AIM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAIM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AIM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AIM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Interferon-inducible protein AIM2 microplate
• Human Interferon-inducible protein AIM2 standard
• Human Interferon-inducible protein AIM2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Interferon-inducible protein AIM2 (AIM2) ELISA Kit has high sensitivity and excellent specificity for detection of Human AIM2. No significant cross-reactivity or interference between Human AIM2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: AIM2 plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2. AIM2 was expressed as a 39-kD protein following in vitro transcription/translation in rabbit reticulocyte lysate or transfection in a murine epithelial cell line. Cell fractionation showed that AIM2 localized primarily to the cytoplasm of transfected mouse cells. Chromosome alterations in malignant melanoma most frequently include translocations and deletions on chromosome 1 or 6 . AIM2 encodes a deduced 344-amino acid protein that contains a conserved sequence domain of approximately 200 amino acids shared with interferon-inducible genes as well as a tyrosine kinase phosphorylation site.

Alternative Names: AIM2; PYHIN4;

Search name: AIM2; PYHIN4;

Tag: AIM2