Human Interleukin 2 antibody (IL2-Ab) ELISA Kit | AE62464HU

Human Interleukin 2 antibody (IL2-Ab) ELISA Kit | AE62464HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: IL2-Ab

Alternative Name: N/A

Application: ELISA

Range: 15.6-1000 pg/mL

Sensitivity: 5.9 pg/mL

Intra-Assay: ≤3.9%

Inter-Assay: ≤7.1%

Recovery: 0, 89

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Competitive ELISA

Analysis Method : Qualitative

Test Principale: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL2-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated IL2-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled IL2-Ab and unlabeled IL2-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of IL2-Ab in the sample. The color development is stopped and the intensity of the color is measured.

Product Overview: Interleukin 2 (IL-2) is a pleiotropic cytokine produced primarily by mitogen- or antigen-activated T lymphocytes. IL-2 plays a key role in promoting the clonal expansion of antigen-specific T cells. In addition, IL-2 has also been shown to mediate multiple immune responses on a variety of cell types.IL-2 stimulates the proliferation of thymocytes; stimulates the proliferation and differentiation of activated B cells; promotes the growth, differentiation and cytocidal activity of monocytes; induces the growth of and cytokine production and cytolytic activity by natural killer cells; enhances the production of lymphocyte-activated killer (LAK) cells; and induces the proliferation and differentiation of oligodendrocytes .

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .