Human IRF4 (Interferon Regulatory Factor 4) ELISA Kit | ELK9110

Human IRF4 (Interferon Regulatory Factor 4) ELISA Kit | ELK9110 | Gentaur UK, US & Europe Distribution

Alternative Names: Interferon regulatory factor 4; IRF 4; IRF-4; Irf4; IRF4_HUMAN; LSIRF; Lymphocyte specific interferon regulatory factor; Lymphocyte specific IRF; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1; MUM 1; MUM1; NF EM5; NF-EM5; NFEM5; PU.1 interaction partner; Sfpi1/PU.1 interaction partner; Transcriptional activator PIP

Species: Human

Assay Type: Sandwich

Sensitivity: 0.122 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Regulatory Factor 4 (IRF4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interferon Regulatory Factor 4 (IRF4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Regulatory Factor 4 (IRF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Regulatory Factor 4 (IRF4) in the samples is then determined by comparing the OD of the samples to the standard curve.