Human ITGa2B (Integrin Alpha 2B) ELISA Kit | ELK4630

Human ITGa2B (Integrin Alpha 2B) ELISA Kit | ELK4630 | Gentaur UK, US & Europe Distribution

Alternative Names: CD41; CD41B; ITG-A2B; GTA; GP2B; GPIIb; HPA3; Platelet Glycoprotein IIb Of IIb/IIIa Complex; Antigen CD41

Species: Human

Assay Type: Sandwich

Sensitivity: 0.055 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Alpha 2B (ITGa2B) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Integrin Alpha 2B (ITGa2B) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Alpha 2B (ITGa2B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Integrin Alpha 2B (ITGa2B) in the samples is then determined by comparing the OD of the samples to the standard curve.