Human ITIH3 (Inter Alpha-Globulin Inhibitor H3) ELISA Kit | ELK3565

Human ITIH3 (Inter Alpha-Globulin Inhibitor H3) ELISA Kit | ELK3565 | Gentaur UK, US & Europe Distribution

Alternative Names: H3P; SHAP; Pre-Alpha (Globulin) Inhibitor, H3 Polypeptide; Serum-derived hyaluronan-associated protein

Species: Human

Assay Type: Sandwich

Sensitivity: 2.39 ng/mL

Standard: 400 ng/mL

Detection Range: 6.25-400 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inter Alpha-Globulin Inhibitor H3 (ITIH3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Inter Alpha-Globulin Inhibitor H3 (ITIH3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inter Alpha-Globulin Inhibitor H3 (ITIH3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inter Alpha-Globulin Inhibitor H3 (ITIH3) in the samples is then determined by comparing the OD of the samples to the standard curve.