Human Kallistatin (SERPINA4) ELISA Kit | AE20056HU

Human Kallistatin (SERPINA4) ELISA Kit | AE20056HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: SERPINA4

Alternative Name: KAL; KLST; KST; PI4; kallistatin; protease inhibitor 4 (kallistatin) |serine (or cysteine) proteinase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin) ; member 4

Application: ELISA

Range: 6.25-400 ng/mL

Sensitivity: 2.46 ng/mL

Intra-Assay: ≤6.2%

Inter-Assay: ≤8.6%

Recovery: 1, 02

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate SERPINA4 in samples. An antibody specific for SERPINA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINA4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Kallistatin is a serine proteinase inhibitor (serpin) and a heparin-binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function.The 1, 284-bp cDNA encodes 427 amino acid residues, including a 26-residue signal peptide and a 401-residue mature peptide. The translated amino acid sequence of kallistatin matches the protein sequence and shares 44 to 46% sequence identity with human alpha-1-antichymotrypsin (AACT), alpha-1-antitrypsin (PI), corticosteroid-binding globulin (CBG), thyroxine-binding globulin of serum (TBG), and protein C inhibitor (PCI) . All of these except TBG, which is encoded by the X chromosome, are encoded by genes on chromosome 14.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .