Human Kappa-casein (CSN3) ELISA Kit | AE58976HU

Human Kappa-casein (CSN3) ELISA Kit | AE58976HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: CSN3

Alternative Name: CSN10; CSNK; KCA;

Application: ELISA

Range: 3.12-200 ng/mL

Sensitivity: 1.31 ng/mL

Intra-Assay: ≤6.9%

Inter-Assay: ≤11.7%

Recovery: 1, 03

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate CSN3 in samples. An antibody specific for CSN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCSN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CSN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CSN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: KCA gene extends over 8, 821 nucleotides and consists of 5 exons ranging from 33 to 496 nucleotides separated by introns ranging from 1, 146 to 2, 942 nucleotides. The human and bovine KCA genes are very similar, with a conserved gene organization and observed identities of 81% and 87% for exons 1 and 2, respectively. The human alpha-S1-casein, beta-casein, and kappa-casein genes are closely linked and arranged in that order. The distance between the alpha-S1 and beta and between alpha-S1 and kappa is approximately 10 and 300 kb, respectively. The beta-casein gene is oriented in the opposite direction to the alpha-S1 and kappa genes. By fluorescence in situ hybridization, the casein gene family was localized to 4q21.1.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .