Human KCNJ10 (Potassium Inwardly Rectifying Channel Subfamily J, Member 10) ELISA Kit | ELK7412

Human KCNJ10 (Potassium Inwardly Rectifying Channel Subfamily J, Member 10) ELISA Kit | ELK7412 | Gentaur UK, US & Europe Distribution

Alternative Names: Kir4.1; Kir1.2; ATP-sensitive inward rectifier potassium channel 10; ATP-dependent inwardly rectifying potassium channel Kir4.1; Inward rectifier K (+) channel Kir1.2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.58 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Potassium Inwardly Rectifying Channel Subfamily J, Member 10 (KCNJ10) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Potassium Inwardly Rectifying Channel Subfamily J, Member 10 (KCNJ10) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Potassium Inwardly Rectifying Channel Subfamily J, Member 10 (KCNJ10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Potassium Inwardly Rectifying Channel Subfamily J, Member 10 (KCNJ10) in the samples is then determined by comparing the OD of the samples to the standard curve.