Human Kinesin-like protein KIF14 (KIF14) ELISA Kit | KTE61920

Human Kinesin-like protein KIF14 (KIF14) ELISA Kit | KTE61920 | Gentaur UK, US & Europe Distribution

Application: This Human Kinesin-like protein KIF14 (KIF14) ELISA Kit employs a two-site sandwich ELISA to quantitate KIF14 in samples. An antibody specific for KIF14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKIF14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KIF14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIF14 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Kinesin-like protein KIF14 microplate
• Human Kinesin-like protein KIF14 standard
• Human Kinesin-like protein KIF14 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Kinesin-like protein KIF14 (KIF14) ELISA Kit has high sensitivity and excellent specificity for detection of Human KIF14. No significant cross-reactivity or interference between Human KIF14 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: KIF14 is a member of the kinesin superfamily of microtubule-associated motors that play important roles in intracellular transport and cell division .KIF14 protein contains an internal motor domain with a conserved catalytic core, an N-type conserved neck region, a forkhead-associated (FHA) domain, and 4 coiled-coil domains. Immunofluorescence microscopy localized KIF14 throughout the cell cycle in 293T cells and HeLa cells. During interphase, KIF14 localized to the cytoplasm and was redistributed to the nucleus upon entry into mitosis. From prophase to metaphase, KIF14 localized at developing spindle poles and associated microtubules, and during anaphase, KIF14 accumulated at the spindle midzone, where it concentrated during telophase. KIF14 localized to the contractile ring in cells ready to undergo abscission.

Alternative Names: KIF14; KIAA0042; MGC142302;

Search name: KIF14; KIAA0042; MGC142302;

Tag: KIF14