Human KiSS-1 receptor (KISS1R) ELISA Kit | KTE61913

Human KiSS-1 receptor (KISS1R) ELISA Kit | KTE61913 | Gentaur UK, US & Europe Distribution

Application: This Human KiSS-1 receptor (KISS1R) ELISA Kit employs a two-site sandwich ELISA to quantitate KISS1R in samples. An antibody specific for KISS1R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKISS1R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KISS1R is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KISS1R bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human KiSS-1 receptor microplate
• Human KiSS-1 receptor standard
• Human KiSS-1 receptor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human KiSS-1 receptor (KISS1R) ELISA Kit has high sensitivity and excellent specificity for detection of Human KISS1R. No significant cross-reactivity or interference between Human KISS1R and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Kisspeptin is encoded by the metastasis suppressor gene KISS1, which is expressed in a variety of endocrine and gonadal tissues. Activation of the kisspeptin receptor is linked to the phospholipase C and inositol trisphosphate second messenger cascades inside the cell. Kisspeptin is involved in the regulation of endocrine function and the onset of puberty, with activation of the kisspeptin receptor triggering release of gonadotropin-releasing hormone (GnRH), and release of kisspeptin itself being inhibited by oestradiol but enhanced by GnRH. Reductions in kisspeptin levels with age may conversely be one of the reasons behind age-related declines in levels of other endocrine hormones such as luteinizing hormone.

Alternative Names: KISS1R; AXOR12; GPR54; HOT7T175; G protein-coupled receptor 54; metastin receptor

Search name: KISS1R; AXOR12; GPR54; HOT7T175; G protein-coupled receptor 54; metastin receptor

Tag: KISS1R