Description
Human KNS2 (Kinesin 2) ELISA Kit | ELK4057 | Gentaur UK, US & Europe Distribution
Alternative Names: KLC; KLC1; KNS2A; hKLC1S; hKLC1N; hKLC1P; hKLC1G; hKLC1R; hKLC1J; hKLC1B; Kinesin Light Chain 1
Species: Human
Assay Type: Sandwich
Sensitivity: 0.106 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Neuro science
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kinesin 2 (KNS2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Kinesin 2 (KNS2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kinesin 2 (KNS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kinesin 2 (KNS2) in the samples is then determined by comparing the OD of the samples to the standard curve.