Human KRT2 (Keratin 2) ELISA Kit | ELK3602

Human KRT2 (Keratin 2) ELISA Kit | ELK3602 | Gentaur UK, US & Europe Distribution

Alternative Names: KRT2A; CK2; KRTE; Cytokeratin 2; Keratin 2A; Keratin 2E; Epidermal Ichthyosis Bullosa Of Siemens; Keratin, type II cytoskeletal 2 epidermal; Epithelial keratin-2e

Species: Human

Assay Type: Sandwich

Sensitivity: 0.056 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Tumor immunity;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 2 (KRT2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Keratin 2 (KRT2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 2 (KRT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 2 (KRT2) in the samples is then determined by comparing the OD of the samples to the standard curve.