Human Krueppel-like factor 5 (KLF5) ELISA Kit | KTE61904

Human Krueppel-like factor 5 (KLF5) ELISA Kit | KTE61904 | Gentaur UK, US & Europe Distribution

Application: This Human Krueppel-like factor 5 (KLF5) ELISA Kit employs a two-site sandwich ELISA to quantitate KLF5 in samples. An antibody specific for KLF5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLF5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLF5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLF5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Krueppel-like factor 5 microplate
• Human Krueppel-like factor 5 standard
• Human Krueppel-like factor 5 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Krueppel-like factor 5 (KLF5) ELISA Kit has high sensitivity and excellent specificity for detection of Human KLF5. No significant cross-reactivity or interference between Human KLF5 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Krueppel-like factor 5 is a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, it is thought to be a transcription factor. Klf5, also known as (IKLF) or (Bteb2) has been assigned purely transcriptional activation activity (Fig. 1A) but, similar to Klf4, binds p300 which acetylates the first zinc finger conferring a trans-activating function. Importantly for Klf4 & Klf5, the amino acids that are predicted by the Klevit model to interact with DNA are identical (Fig. 1B and Fig. 2) and the two compete for the same CACCC element found in a wide variety of promoters.

Alternative Names: KLF5; BTEB2; CKLF; IKLF; GC box binding protein 2; Kruppel-like factor 5; basic transcription element binding protein 2; colon kruppel-like factor; intestinal-enriched kruppel-like factor; transcription factor

Search name: KLF5; BTEB2; CKLF; IKLF; GC box binding protein 2; Kruppel-like factor 5; basic transcription element binding protein 2; colon kruppel-like factor; intestinal-enriched kruppel-like factor; transcription factor

Tag: KLF5