Human L-lactate dehydrogenase B chain (LDHB) ELISA Kit | KTE61841

Human L-lactate dehydrogenase B chain (LDHB) ELISA Kit | KTE61841 | Gentaur UK, US & Europe Distribution

Application: This Human L-lactate dehydrogenase B chain (LDHB) ELISA Kit employs a two-site sandwich ELISA to quantitate LDHB in samples. An antibody specific for LDHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLDHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LDHB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LDHB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human L-lactate dehydrogenase B chain microplate
• Human L-lactate dehydrogenase B chain standard
• Human L-lactate dehydrogenase B chain detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human L-lactate dehydrogenase B chain (LDHB) ELISA Kit has high sensitivity and excellent specificity for detection of Human LDHB. No significant cross-reactivity or interference between Human LDHB and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: LDHB and peptidase B are linked (Santachiara et al., 1970) and both loci are on chromosome 12 (Chen et al., 1973) . Kitamura et al. (1971) reported the first case of a complete deficiency of lactate dehydrogenase subunit H (B) in serum, saliva and erythrocytes of a 64-year-old male with mild diabetes. Study made on family members revealed low LDH activity in their serum also linked with decreased relative activity of the H4 (B4) fraction. Based on the comparison of the calculated ratio of H to M subunits in normal and affected family members, it was hypothesized that the proband is homozygous while the abnormal family members are heterozygous, assuming a single gene is involved. Red cell metabolism in the proband was studied by Miwa et al. (1971) ; neither reticulocytosis nor hemolytic anemia was present.

Alternative Names: LDHB; LDH-H; TRG-5; L-lactate dehydrogenase B; OTTHUMP00000165229

Search name: LDHB; LDH-H; TRG-5; L-lactate dehydrogenase B; OTTHUMP00000165229

Tag: LDHB